Crystallization, X-ray diffraction analysis and phasing of 17β-hydroxysteroid dehydrogenase from the fungusCochliobolus lunatus
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Purification, crystallization and preliminary X-ray diffraction analysis of aspartate semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis.
Aspartate semialdehyde dehydrogenase from Mycobacterium tuberculosis (Asd, ASADH, Rv3708c), which is the second enzyme in the lysine/homoserine-biosynthetic pathways, has been expressed heterologously in Escherichia coli. The enzyme was purified using affinity and gel-filtration chromatographic techniques and crystallized in two different crystal forms. Preliminary diffraction data analysis sug...
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In the paper by Audette et al. [Acta Cryst. (1999), D55, 1584-1585] the postal code of one of the authors was printed incorrectly. The correct version is given above. Also the reaction catalyzed by isocitrate dehydrogenase was given incorrectly in the paper; the correct reaction is given below. Isocitrate + NADP(+) <--> Oxalosuccinate + NADPH. Oxalosuccinate <--> alpha-Ketoglutarate + CO(2).
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications
سال: 2005
ISSN: 1744-3091
DOI: 10.1107/s1744309105034949